The Laboratory of Vegetal Biotechnologies

The head of the lab: Maria Liliana, PhD in biology, associate professor (docent)

Research team: 15 people, including 10 researchers, 4 PhD in biology

Smerea Svetlana, dr.biol., cercet. şt. coord.  [email protected]
Andronic Larisa, dr.hab. biol., cercet. şt. coord. [email protected]
Mărîi Liliana, dr.biol., cercet. şt. coord. [email protected]
Saltanovici  Tatiana, dr.biol., cercet. şt. coord. [email protected]
Antoci Ludmila, cercetător ştiinţific
Grigorov Tatiana, cercetător ştiinţific
Chitrosan Liliana, cercetător ştiinţific  stagiar   
Erhan Irina, cercet. ştiinţific stagiar
Racu Vadim, cercet. ştiinţific stagiar
Doncila Ana, cercet. ştiinţific stagiar
Ursachi Olga, biolog/farmacist
Amarfii Vera, laborant superior
Brînzan Alexandr, inginer coordonator
Gradinaru Ion, inginer coordonator
Macovei Ecaterina, doctorand

 

Research directions:

  • Manipulation of the recombination process (identification of critical meiotic stages against viral infections, evaluation of the particularities of meiotic crossing-over and the spectrum of aberrations in meiosis in conditions of viral pathogenesis);
  • Variability of quantitative and qualitative traits in offspring derived from parents infected with viruses and / or irradiated with gamma barley (Hordeum vulgare L.) and tomatoes (Solanum lycopersicum L., S. cheesmaniae, S. pimpinellifolium);
  • The cytogenetic specificity of the host-pathogen interaction (histological and ultrastructural aspects, mutational and recombinogenic effects induced in plants, the specificity of the crossingover rate);
  • Application of gametic selection methods in evaluating and identifying the potential resistance of genotypes to environmental factors (viral infections, supraoptimal temperatures, water stress) at the level of male gametophyte in order to streamline the selection process and assess the potential for adaptability.
  • Plant biotechnologies:

    •    induction and evaluation of somaclonal variations generated by tissue cultures of cultivated species (potato, cabbage, wheat, spring barley);
    •    regeneration of haploid plants by in vitro culture of anthers and ovaries (spring barley);
    •    obtaining and multiplying virus-free material (potato, vine);
    •    in vitro rescue technology for immature embryos in the perennial vine varieties;
    •    in vitro micropropagation (Vitis vinifera, Solanum tuberosum, Lavandula angustifolia, Satureja montana, Stevia rebaudiana, Paulownia etc.)
     

    Scientific skills:

  • Application of in vitro technologies in the creation of valuable forms of different crops, especially cereals (wheat and spring barley).
  • The use of in vitro techniques and experimental mutagenesis based on the application of viruses as a factor for inducing variability and / or gamma radiation, with the possibility of obtaining new forms of crops (tomatoes, barley and wheat) with valuable agronomic characteristics.
  • Obtaining and multiplying virus-free plants. Based on research conducted over 15 years, algorithms have been developed to obtain healthy material for a number of crops - potato, lavender, stevia and vines.
  • Virological diagnosis of vegetative material:
    1. enzyme-linked immunosorbent assay (ELISA)
    2. immunosorbent electron microscopy (ISEM)
    3. electron contrast microscopy with negative contrast


     

    Relevant results:

    1. It has been established that infections caused by tomato mosaic, tomato aspermia and potato X viruses induce:

    complimented changes in the distribution and exchange rate between homologous chromosomes, significant deviations of mitosis and meiosis processes in host plants.
    2. The assumption regarding the mitogenic effect of phytoviruses was confirmed by the SCE (sister chromatid exchange) test on grain and spring barley.
    3. Experimental mutagenesis methods, based on the use of phytoviruses as inducers of variations, based on different host-pathogen systems, have proven the possibility of expanding genetic diversity, thus obtaining new forms of crop plants (tomatoes, wheat, barley spring), showing positive or negative transgressions.
    4. Based on cytological studies, the restructuring induced by viral infections in the organs of the reproductive system in different crops (tomatoes, wheat, spring barley) was described, being appreciated the reactivity at tissue and cellular level compared to the reorganizations generated by gamma rays.
    5. The optimization of in vitro cultivation conditions and nutrient media allowed the development of working algorithms for micropropagation and ex vitro acclimatization of several plant species of ameliorating interest (Solanum tuberosum, Lavandula angustifolia, Satureja montana, Stevia rebaudiana, Vitis vinifera, Paulownia sp).
    6. In order to obtain the propagating material free of viral infections, the protocols for the eradication of viral twins were prepared by meristem culture with or without thermotherapy.
    7. The application of the in vitro culture technique of immature embryos allowed the elaboration of the procedure for the recovery of the vine seedlings with different degree of apirenia and eradication in this way of the viral germs of the degenerative complex.
    8. For different plant-virus interaction systems (compatible, incompatible, sensitive, tolerant, resistant), transgenerative features of the defensive response of plants were highlighted.

     

    Innovative aspects:

    The innovative aspect is confirmed by 8 patents, inclusive:  

  • ANDRONIC, L. Procedeu de sporire a variabilităţii  somaclonale la varză. Brevet de invenţie MD 461,  2012-01-31.
  • ANDRONIC, L.; GRIGOROV, T.; BUJOREANU, V. Procedeu de obţinere a recombinanţilor  de orz de primăvară. Brevet de invenție MD 6507. 2010-06-07.
  • ANDRONIC, L;.JACOTĂ A.; BUJOREANU, V. Procedeu de obţinere a recombinanţilor de tomate.  Brevet de invenție MD 6403. 2010-03-18.
  • SMEREA, S.; ANDRONIC, L. Procedeu de obținere a plantelor de viță de vie libere de virusul răsucirii frunzelor. MD 847 Z 2015.07.31. (implementat).
  • SMEREA, S.; ANDRONIC, L.; MACOVEI, E.; MAȘCENCO, N. Procedeu de inducere a haploizilor la orz. MD 1090 2017.06.30.

  • Experience of collaboration with other national research organizations, economic agents in the country:

    1.    S.C. IMUNOTEHNOMED S.R.L. Domeniul de colaborare: Consultanță științifică pentru pregătirea probelor din material vegetal pentru diagnosticul virusologic prin tehnici ELISA.
    2.    SRL Bioplant. Domeniul de colaborare: Aplicarea procedeelor de microscopie electronică în expertiza virusologică a materialului săditor de nuc (contract tehnico-științific).
    3.    SRL Business Protect. Domeniul de colaborare: Multiplicarea microclonală a plantulelor de Paulownia (contract tehnico-științific).

    Contacts:

    Tel.:  (+ 373 22) 66 03 89
    Fax:  (+373 22) 55 61 80
    Mărîi Liliana, dr.biol., cercet. şt. coord. [email protected]

    Patent for plant variety

    1.    MĂRÎI, L.; BUJOREANU, V.; ANDRONIC, L. ; SMEREA, S. ; BOTNARI, V. Soi de tomate Anona. MD 276 2018.10.31.
    2.    MĂRÎI, L.; BUJOREANU, V.; ANDRONIC, L. ; SMEREA, S. ; BOTNARI, V. ; BERZOI, V. Soi de tomate CisGen. MD 277 2018.10.31.

    Certificate for plant variety

    1.    MĂRÎI, L., BUJOREANU, V., ANDRONIC, L., SMEREA, S., BOTNARI, V., BERZOI, V. Soi de tomate CisGen. Nr.717. 2018.

    Relevant scientific publications

    Monographs

    1.    ANDRONIC, L. Bazele citogenetice ale variabilității genetice la plantele de cultură în condiții de patogeneză virală. Ch.: Print-Caro, 2020, 236 p. ISBN 978-9975-56-762-6.

    Teaching papers

    2.    ANDRONIC, L. Biologie celulară (note de curs). Red. DUCA M. Chi.: Biotehdesign, 2017. 207 p. ISBN 978-9975-108-21-8.

    Articles from journals with impact factor

    3.    САЛТАНОВИЧ Т.И., ДОНЧИЛЭ А.Н. Идентификация  устойчивых генотипов томата с использованием гаметных технологий. VII международная научно-практическая конференция: «Современные тенденции в селекции, семеноводстве и товарном производстве овощных, бахчевых и цветочных культур. Традиции, современность, перспективы». Овощи России. N4. 2020. p.50-54.  ISSN 2072-9146.( IF – 0,415)
    4.    IVANOVA, R.; SMEREA, S. Safflower yield response to irrigation and gamma irradiation. Agriculture and and forestry. 2019, 65(1), 29-38. eISSN 1800-6492; pISSN 0554-5579. doi: 10.17707/AgricultForest.65.1.03 (IF: 1.2545).
    5.    ANDRONIC, L.; SMEREA, S.; MACOVEI, E. In vitro response of anthers from different barley genotypes in order to obtain haploid plants. Romanian Agricultural Research. 2015, 32, 3-10. Print ISSN 1222-4227; Online ISSN 2067-5720. (IF: 0.28).
    6.    ANDRONIC L. Viruses as trigger of DNA damage in host plants. Canadian Journal of Plant Science, 2012, 92 (6), p.1083-1091.
    7.    ANDRONIC L., JACOTA A.G., BUJOREANU V.V., GRIGOROV T.B. Genotoxicity of barley stripe mosaic virus in infected host plants. Central European Journal of Biology, 2010, 5(5), p.633-640.

    Articles from other magazines published abroad

    8.    SMEREA, S.; ANDRONIC, L. The increasing of the in vitro multiplication rate for grapevine genotypes by application of low-intensity millimeter waves. Lucrări şt. Univ. de Ştiinţe Agricole şi Medicină Veterinară „Ion Ionescu de la Brad”. Ser. Horticultură. Iași. 2015, 58(2), 45-50. ISSN 1454-7376. (categoria B+, România).
    9.    АНДРОНИК, Л.И.; БАЛАШОВА, И.Т.; БУЖОРЯНУ, В.В.; СМЕРЯ, С. В. Специфические реакции разных генотипов томата  Solanum lycopersicum L. на заражение вирусами. Плодоводство и ягодоводство России, 2017, том XXXXVIII, часть I. c. 17-22, ISSN 2073-4948.
    10.    SMEREA, S.; ANDRONIC, L.; SCHIN, V. Callusogenetic and morphogenetic capacity of safflower explants. Lucrări şt. Univ. de Ştiinţe Agricole şi Medicină Veterinară „Ion Ionescu de la Brad”. Ser. Horticultură. Iași. 2017, 60(2), 33-38. ISSN 1454-7376. (categoria B+, România).
    11.    SMEREA, S.; ANDRONIC, L.; SCHIN, V. Morphogenetic potential of calluses derived from gamma irradiated safflower seeds. Muzeul Olteniei Craiova. Oltenia. Studii și comunicări. Științele Naturii. 2018, 34(2), 67-71. ISSN 1454-6914. (categoria B+, România).
    12.    IVANOVA, R.; SMEREA, S. Effects of seeds irradiation with gamma-ray on plant growth and yield attributing characters of safflower. Oltenia. Studii și comunicări. Științele Naturii. Muzeul Olteniei Craiova. 2018, 34(2), 51-56. ISSN 1454-6914. (categoria B+, România).

    Articles in national journals, category B

    13.    ANDRONIC, L.; MACOVEI, E.; SMEREA, S. Ultrastructural evaluation and histological peculiarities of androgenetic structures of barley. Buletinul Academiei de Științe a Moldovei Ştiinţele vieţii. 2015, nr. 2(326), 53-58. ISSN 1857-064X.
    14.    ANDRONIС, L.; BALASHOVA, I.; BUJOREANU, V.; SMEREA, S. Some reactions to virus infections of Solanum lycopersicum L. genotypes with different defense response. Conferinţa ştiinţifică internațională „Genetica, fiziologia şi ameliorarea plantelor”, 9-10 octombrie 2017, Chişinău. p. 102-105. ISBN 978-9975056-463-2.

    Articles in international collections

    15.    ANDRONIC, L. I.; SMEREA, S. I.; VOINU, M.  Phenotypic expression and variance of quantitative traits in tomato progenies obtained from tomato plants infected with viruses.  Collection of articles of International Conference ''Agrophysics trends: from actual challenges in arable farming and crop growing towards advanced technologies", Saint Petersburg, 27 - 29 September 2017. p. 422-426. ISBN 978-5-905200-34-2.
    16.    ANDRONIC, L. Influence of viral infection on meiotic progression in tomatoes cultivars. В: Современные тенденции в селекции и семеноводстве овощных культур. Традиции и перспективы. Материалы научно-практической конференции ВНИИСОК, Москва, 2015, c. 62-69. ISBN 978-5-901695-65-4.
    17.    ANDRONIC, L.; MARII, L.; SMEREA, S.; SCHIN, V. Specific effects of viral infection in tomato progenies obtained from plants infected with viruses. The novel results of the Institute of Biology Bucharest into fields of ecology, microbiology and citobio¬logy. București: Ars Docendi, 2018 p.14-21. ISBN 978-606-998-044-6.
    18.    CМЕРЯ, C. Зависимость регенерационной способности томата in vitro от генотипа и типа экспланта в состоянии вирусного патогенеза. В сборнике материалов конференции «Тенденции развития агрофизики: от современных проблем земледелия и растениеводства к технологиям будущего». 27-29 сентября, 2017, Санкт-Петербург. c. 374-378. ISBN 978-5-905200-34-2.
    19.    АНДРОНИК, Л. Экспрессия некоторых генов ячменя в потомстве вирус- инфицированных родительских форм.  Международная научно-практическая конференция, «Фундаментальные и прикладные исследования в биоорганическом сельском хозяйстве России, СНГ и ЕС», Москва, 9-12 августа 2016, c. 92-101. ISBN 978-5-98467-015-9.

    20.    ANDRONIC, L.; SMEREA, S.; MARII, L. Variance of quantitative traits in tomato progenies obtained from tomato plants infected with viruses. In: Роль физиологии и биохимии в интродукции и селекции сельскохозяйственных растений V Международная научно-методологическая конференция, 15-19 апреля 2019 г., Москва, Том II, с.7-11, ISBN 978-5-209-09359.

    Participation in exhibitions / shows
    In the last 5 years over 20 participations, with 14 gold medals

    1.    SMEREA, S.; ANDRONIC, L. Procedeu de multiplicare microclonală a viţei de vie. Salonul Internațional de Invenții și Inovații 'Traian Vuia', Ediția a II-a, 25-27 mai 2016, Timișoara, România. Catalog oficial, p.38. (Medalia de aur).
    2.    SMEREA, S., ANDRONIC, L., MACOVEI, E. Procedeu de inducere a haploizilor la orz.  Expoziția Europeană de Creativitate și Inovație, EUROINVENT, 2017, Ediția a IX-a, 25-27 mai, Iași, România. Catalog oficial, p.202. ISBN: 978-606-775-212-0. (Medalie de aur).
    3.    MĂRÎI, L., BUJOREANU V., ANDRONIC, L., SMEREA, S., BOTNARI V., BERZOI V. CisGen - Soi nou de tomate. PRO INVENT 2018. Salonul Internațional al cercetării științifice, inovării și inventicii. Ediția a XVI-a. 21 – 23 martie 2018, Cluj-Napoca, România. Catalog oficial, p.47. ISBN 978-606-737-288-5. (Diplomă de excelență și Medalie de aur).
    4.    MĂRÎI, L.; BUJOREANU, V.; ANDRONIC, L.; SMEREA, S.; BOTNARI, V. Anona – new tomato cultivar. Expoziția Europeană de Creativitate și Inovație, EUROINVENT, 2019, Ediția a XI-a, 16-18 mai, Iași, România. Catalog oficial p.226-227. ISSN Print: 2601-4564; Online: 2601-4572. (Medalie de aur).

    Participation in projects

    1.    INTAS Nr. 05-104-7654 “Utilizarea marcherilor moleculari în selecţia noilor genotipuri  de viţă de vie cu caractere de apirenie şi rezistenţă la condiţiile de mediu” (perioada de realizare: 2006-2008).
    2.    STCU 4073 „Crearea colecţiei de forme locale de  viţă  de vie libere de virusuri cu rezistentă la factori de mediu” (perioada de realizare: 2008-2011).
    3.    STCU 5383 „Embriogeneza zigotică – procedeu biotehnologic eficient de eradicare a virusurilor la soiurile apirene de viţă de vie” (perioada de realizare: 2012-2013).
    4.    STCU 6097 “Cercetări biologice, chimice și biotehnologice ale plantelor de Carthamus spp. (șofrănel)”, (perioada de realizare: 2016-2018) fiind responsabili de  studiul morfo-fiziologic și biochimic al șofrănelului obținut prin cultura in vitro  cu aplicarea mutagenezei experimentale.
    5.    Proiect de asistență tehnică ”Îmbunătățirea securității și controlului materialelor radioactive în baza Programului Internațional de Reducere a Amenințărilor Radiologice”, (perioada de realizare: 2016-2019).

    The laboratory's researchers have become members of the European Association for Science and Technology Cooperation (COST) under the EU's Horizon 2020 Research and Innovation Program, actions:

    1.    CA16212 - Impact of Nuclear Domains on Gene Expression and Plant Traits;
    2.    CA18127 - International Nucleome Consortium;

    3.    CA19125 - EPIgenetic mechanisms of Crop Adaptation to Climate Change.

    List of collaborations

    1. Institute of Biology Bucharest, Romania
    2. Horticultural Improvement Research Institute, Quedlinburg, Germany
    3. Institute for Scientific Research in the Improvement and Production of Vegetable Seeds, Odintsovo, Moscow, Russian Federation
    4. Institute of Agrophysics, Saint Petersburg, Russian Federation


     

Institute of Genetics, Physiology and Plant Protection

20 Padurii str., MD-2002, Chisinau, Republic of Moldova, Tel.: (+373 22) 77-04-47, Fax: (+373 22) 55-61-80, E-mail: [email protected]

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