The head of the lab: Dascaliuc Alexandru, dr. hab., prof. univ.
Research team:
1. Calugaru-Spataru Tatiana, doctor of biological sciences,head of the laboratory
2. Dascaliuc Alexandru, doctor habilitat in biological sciences, university professor, scientific consultant
3. Balaur Nicolae, doctor habilitat in biological sciences, corresponding member of the ASM, scientific consultant
4. Caus Maria, doctor of biological sciences, coordinating scientific researcher
5. Ralea Tudor, doctor of biological sciences, coordinating scientific researcher
6. Călugăru-Spătaru Tatiana, doctor of biological sciences, senior scientific researcher
7. Jelev Natalia, scientific researcher
8. Platovschii Nicolai, scientific researcher
9. Badașco Sabina, scientific researcher
10. Zdioruk Nina, trainee scientific researcher
11. Delean Tatiana, physiologist cat. and
12. David Diana, chemist, maternity leave
Research directions:
• Determining the parameters that characterize the resistance of plants with different levels of organization to the action of extreme temperatures;
• biosynthesis of secondary metabolites in vivo and in vitro;
• determining the role of secondary metabolites in the adaptation of plants to stressors.
• elaboration and testing of the biological activity of biostimulators.
Scientific skills:
• Elaboration of accelerated methods for determining the resistance and adaptability of plants with different levels of organization to the action of heat and frost;
• Obtaining and implementing natural growth regulators to increase resistance and restore damage caused by exposure of different plant species to high temperatures and frost;
• the study of the composition and accumulation of secondary metabolites in different plant species and the determination of the factors that ensure the increase of the content of the active principles; obtaining secondary metabolites in in vitro and in vivo culture;
• micropropagation of different plant species (Rhodiola rosea, Actinidia arguta, Gerbera jamesonii, Lilium martagon, Mentha gattefossei, etc.) under in vitro conditions.
Relevant results:
• Accelerated methods have been developed to determine the primary resistance (basic resistance) and adaptability (acclimatization) of plants to high temperatures and frost. The practical use of the mentioned methods gave the possibility to distribute different wheat varieties according to their primary resistance to high temperatures and frost and to highlight the varieties with combined resistance to both frost and high temperatures. In field conditions it has been shown that after the first and second hardening phases, during winter, the distribution of wheat genotypes after final frost resistance corresponds to their distribution according to primary resistance, which suggests the prospects of practical use of developed methods.
• Using the method of accelerated determination of the primary resistance of wheat genotypes at high temperatures and frost, it was shown that the level of primary resistance is substantially influenced by seed reproduction conditions, suggesting that in hexaploid wheat the level of primary resistance to extreme temperatures is determined by the genetic (hard) and epigenetic (labile) inheritance. This fact must be taken into account when carrying out the activities of testing and homologation of wheat varieties.
• It has been shown that treating wheat seeds before sowing with the Reglalg biostimulator not only ensures better adaptation of plants to frost and high temperatures, but also induces an increased resistance to high and negative temperatures to freshly germinated seedlings (aged 2 years). - 3 days). It follows that Reglalg beneficially influences not only the adaptation processes, due to the change in gene expression, but also acts as a protector, directly reducing the negative effects of seedling exposure to the action of extreme temperatures. Due to the mentioned effects, the plants obtained from the seeds treated before sowing with the Reglalg biostimulator, compared to the control plants, adapt faster to extreme temperatures, are more vigorous throughout the vegetation period, which ultimately ensures the increase in quantity and quality. harvest.
• Based on the introduction of cinnamic alcohol and the Reglalg biostimulator into the in vitro culture medium, as well as due to the exposure to low temperatures and UV radiation, of the R. rosea cell culture at the beginning of the exponential growth phase, several stimulation of biomass accumulation and increase the content of secondary metabolites, characteristic for R. rosea rhizomes.
Research equipment and methods:
Innovative aspects:
1. CAUȘ, M.; CĂLUGĂRU-SPĂTARU, T.; DASCALIUC, A. Metodă de determinare a temperaturii de inhibiție a sistemului radicular la castravete Cucumis sativus L. Brevet de invenție MD 1134, 2017.11.30.
2. CAUȘ, M.; CĂLUGĂRU-SPĂTARU, T.; DASCALIUC, A. Procedeu de determinare a sexului la plantele de Actinidia arguta in vitro. Brevet de invenție MD 1055 Z, 2017.02.28.
3. CĂLUGĂRU-SPĂTARU, T.; CAUȘ, M.; DASCALIUC, A. Procedeu de obținere a biomasei calusului de Rhodiola rosea L. in vitro. Brevet de invenție MD 894 Z, 2015.11.30.
4. CĂLUGĂRU-SPĂTARU, T.; CIOCÂRLAN, N.; CAUȘ, M.; DASCALIUC, A. Procedeu de micropropagare a plantelor de Mentha gattefossei Maire in vitro. Brevet de invenție MD 1091, 2017.06.30.
5. CAUȘ M., CĂLUGĂRU-SPĂTARU T., DASCALIUC A. Procedeu de tratare a semințelor de castravete Cucumis sativus L. MD Nr. 1296 Z, 2019.08.31.
Assistance, scientific services provided:
• Methods for determining reactive oxygen species: oximetry, potentiometry, spectrophotometry, electrophoresis.
• Biochemical methods for determining the composition of biological objects: spectrophotometry, centrifugation, chromatography, electrophoresis, determination of the activity and spectra of redox isoenzymes.
• Physiological and biophysical methods for determining the condition of plants: fluorometry, electrolyte leakage, seed germination, plant growth and development, resistance to extreme temperatures, differentiation and dedifferentiation in in vitro and in vivo conditions.
• Artificial methods of induction and modification of plant condition: application of extreme temperatures and drought on plants; the influence of light depending on the spectrum, the influence of phytohormones, the influence of growth regulators and elicitors on the resistance of plants to abiotic stressors.
• Biotechnological methods for introduction and micropropagation of precious plant genotypes under in vitro conditions.
• Methods for the accelerated determination of the primary resistance of plant genotypes to excessive temperatures.
• Methods for assessing the influence of biostimulators on the physiological state and resistance of plants to extreme temperatures.
Relevant publications:
Articles from journals with impact factor 0,1-0,9
1. ЖЕЛЕВ, Н.Н., ДАСКАЛЮК, А.П. Модификация устойчивости растений пшеницы (Triticum aestivum L.) к экстремальным температурам при помощи природного регулятора роста Реглалг. Журнал «Агрохимия», Издательство: Российская академия наук, Москва, 2019, 6, cc. 34-43. ISSN: 0002-1881 (IF: 0.621). doi: 10.1134/S0002188119040136.
Articles from magazines published abroad, category B
1. CAUȘ, M. Changes in acid phosphatase activity and isoform patterns in root cytoplasmic and membrane bound protein fractions of Pisum sativum L. seedlings exposed to cadmium application. Lucrări științifice, Seria Horticultură, 2016, vol. 59, nr.1, pp. 49-55. ISSN 1454-7376. http://www.uaiasi.ro/revista_horti-en/arhiva.php?an=2016
2. CAUȘ, M., DASCALIUC, A. Biostimulant effects of Reglalg on biochemical parameters of horticulture plants”. Lucrări şt. Univ. de Ştiinţe Agricole şi Medicină Veterinară “Ion Ionescu de la Brad”. Ser. Horticultură. Iași, România. 2019, vol. 62, nr.1, pp. 89-94. ISSN: 2069-8275. ISSN 1454-7376. http://www.uaiasi.ro/revista_horti-en/files/arhiva/Vol-62-1_2019.pdf.
3. DASCALIUC, A., JELEV, N. The influence of biostimulant Reglalg on wheat plants resistance to frost. Acta Scientific Agriculture. 2019, 3 (10), pp. 105-108. ISSN 2581-365X. https://actascientific.com/ASAG/pdf/ASAG-03-0656.pdf
Articles in national journals:
1. CAUS, M., DASCALIUC, A. Peroxidase activity in cucumber (Cucumis sativus L.) seedlings obtained from seeds treated with natural growth regulator Reglalg. Journal of Botany. Ch., 2015, vol. VII, nr. 1(10), 10- 16. ISSN 1857-095X.
2. CAUȘ M., DASCALIUC A. Influența biostimuatorului Reglalg asupra reacției plantulelor de castravete (Cucumis sativus l.) la șocul termic. Buletinul AȘM, Științele vieții. 2019, 2(338), pp. 85-95. ISSN: 1857-064X.
3. CAUȘ, M., CĂLUGĂRU-SPĂTARU, T.; DASCALIUC, A. Determinarea accelerată a rezistenței radiculei și rădăcinilor secundare a plantulelor de castravete (Cucumis sativus L.) la temperaturi ridicate. Buletinul Acad. de Științe a Moldovei. Științele vieții. 2017, nr. 2, pp. 49-57. ISSN 1857-064X.
4. CAUȘ, M., CĂLUGĂRU-SPĂTARU, T., DASCALIUC, A. Influența reglatorului natural de creștere Reglalg asupra capacității calusului de Rhodiola rosea L. de a acumula substanțele ce influențează potențialul oxido-reductiv al celulelor. Buletinul Academiei de Științe a Moldovei, Științele vieții. 2016, nr 2 (329), pp. 40-48. ISSN 1857- 064X.
5. CĂLUGARU-SPATARU, T. Effects of natural growth regulator Reglalg on the Rhodiola rosea L. callus growth rate. Journal of Botany. Ch., 2015, vol. VII, Nr. 1(10), pp. 5 – 10. ISSN 1857-095X.
6. CĂLUGĂRU-SPĂTARU, T. Acumularea metaboliților secundari în Rhodiola rosea L. în funcție de condițiile mediului. Buletinul Academiei de Științe a Moldovei, Științele vieții. 2015, nr. 1(325), pp. 85-92. ISSN 1857- 064X.
7. CĂLUGĂRU-SPĂTARU, T. Influența Reglalgului asupra acumulării biomasei și pigmenților fotosintetici în celulele calusului și agregatelor celulare ale rădăcinii aurii (Rhodiola rosea L.). Buletinul Academiei de Științe a Moldovei, Științele vieții. 2017, nr. 1, pp. 39-47. ISSN 1857-064X.
8. CĂLUGĂRU-SPĂTARU, T., CIOCÂRLAN, A., DASCALIUC, A. Compoziția chimică a extractelor și uleiului volatil din rizomii de Rhodiola rosea L. de origine Carpatină. Buletinul Academiei de Științe a Moldovei, Științele vieții. 2017, nr. 3, pp. 78-83. ISSN 1857-064X.
9. DASCALIUC, A., VOINEAC, V., RALEA, T., JELEV, N. The influence of biostimulant Reglalg on plants resistence to abiotic and biotic stress factors. Buletinul AȘM, Științele vieții. 2018, nr. 3(336), pp. 76-82. ISSN 1857-064X.
10. DASCALIUC, A., CUZA, P. Senescența și scurgerea electroliților din frunzele stejarului pediculat (Quercus robur L.) cu diferite termene de înfrunzire. Mediul ambiant, 2015, nr. 2, (80), pp. 20-25. ISSN 1810-9551
11. JELEV, N. Diminuarea impactului factorilor de stres abiotic asupra plantelor de Triticum aestivum L. prin utilizarea regulatorului natural de creștere Reglalg. Buletinul Academiei de Științe a Moldovei, Științele vieții. 2016, nr 3(330), pp. 72-79. ISSN 1857-064X
12. JELEV, N., ZDIORUK, N., RALEA, T., GOREA, A., DASCALIUC, A. Aprecierea accelerată a termotoleranței genotipurilor de grâu în baza specificului influenței șocului termic asupra germinării semințelor. Buletinul Academiei de Științe a Moldovei, Științele vieții. 2015, nr 3(327), pp. 60-66.
13. JELEV, N., ZDIORUK, N., RALEA, T., DASCALIUC, A. Particularitățile germinării semințelor de Triticum Aestivum L. expuse șocului. Buletinul Academiei de Științe a Moldovei, Științele vieții. 2015, nr 3(327), pp. 50-60.
14. JELEV, N., ZDIORUK, N., RALEA, T., DASCALIUC, A. Particularitățile germinării semințelor de Triticum Aestivum L. expuse șocului. Buletinul Academiei de Științe a Moldovei, Științele vieții. 2015, nr 3(327), pp. 50-60.
15. ZDIORUK, N., RALEA, T., JELEV, N., CUZA, P., FLORENTA, G. The seasonal activity of photosystem II and enzimes of degradation of hydrogen peroxide and during dehydration of Buxus sempervirens L. leaves. Journal of Botany. Ch., 2015, vol. VII, nr. 1(10), pp. 27- 31. ISSN 1857-095X.
16. ЖЕЛЕВ, Н., ДАСКAЛЮК, А., РАЛЯ, Т., ЗДИОРУК, N., ОБОЗНЫЙ, А., ПАРИЙ, Ю., ПАРИЙ, Я. Сравнительная оценка первичной устойчивости генотипов пшеницы (Triticum aestivum L.) к положительным и отрицательным температурам. Buletinul Academiei de Științe a Moldovei, Științele vieții. 2018, nr. 1 (334), pp. 61-70. ISSN 1857-064X.
Contacts:
Institute of Genetics, Physiology and Plant Protection, Plant Biochemistry Laboratory
Str. Pădurii, 20, of 221.
Tel: 53-01-77
E-mail: [email protected]